Our main achievements in 2015

1. The structural features of auxin responsive elements were detected. The developed bioinformatical method is uniform and can be used for the study of other molecular-genetics processes with complex regulation.
2. The evolution of auxin biosynthesis pathway in plants revisited. Stoneworts Klebsormidium flaccidum are the only algae in which the homologs of higher plants’ auxin biosynthesis enzymes were found. This fact testified in favor of the hypothesis of vertical inheritance of auxin biosynthesis pathway from multicellular algae to the higher land plants. In collaboration colleagues from ICG we performed comparative analysis of algae, moss and higher plants genomes. Based on the results of the reconstruction of three-dimensional structure of proteins, analysis of domain structure and detailed phylogenetic analysis it was shown that the homologs of auxin biosynthesis enzymes, previously reported in K. flaccidum, are not the ones. The obtained results testify in favor of the hypothesis of horizontal transfer of the auxin biosynthesis genes during the colonization of land by plants. The work was published in Trends in plant science, 2015.
3. The 3D map of PIN1 expression domain in the stem cell nishe of A. thaliana root meristem was described.
In collaboration with Klaus Palme’ lab we perform systems analysis of auxin distribution in the plant root
4. A number of bioinformatical methods and approaches were developed for biological data analysis. In collaboration with experimental biologists, the lab members were developed the methods for transcriptome analysis, SNP distribution, non-coding RNA , 3D chromatin organization. We developed the algorithms and methods of bioinformatic analysis of whole genome markings and its link to the known annotation of genes structure, including proximal and distal parts of promoter, exons and introns of 5’-untranslated region, etc. and methods for prediction of gene translation elongation efficiency (Sokolov et al., Journal of Integrative Bioinformatics, 2015)